Anti-Ms CD197 PE

• Description:

  CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.

• Certification:

  RUO

• Reactivity:

  Mouse

• Immunogen:

  Murine CD197-transfected RBL-2H3 cells

• Target Antigen:

  CD197

• Clone:

  4B12

• Applications:

  FC (QC tested)

• Concentration:

  0.5 mg/mL

• Format:

  PE

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Singh V, Gowthaman U, Jain S, Parihar P, Banskar S, Gupta P, Gupta UD, Agrewala JN: Coadministration of interleukins 7 and 15 with bacille Calmette-Guérin mounts enduring T cell memory response against Mycobacterium tuberculosis. J Infect Dis. 2010 Aug 15;202 (3) :480-9, URL: http://www.ncbi.nlm.nih.gov/pubmed/20569158, *Adachi K, Osada Y, Nakamura R, Tamada K, Hamano S: Unique T cells with unconventional cytokine profiles induced in the livers of mice during schistosoma mansoni infection. PLoS One. 2013 Dec 16;8 (12) :e82698., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=adachi+k+osada+y , *Huggins A, Paschalidis N, Flower RJ, Perretti M, D'Acquisto F: Annexin-1-deficient dendritic cells acquire a mature phenotype during differentiation. FASEB J. 2009 Apr;23 (4) :985-96, URL: http://www.ncbi.nlm.nih.gov/pubmed/19029200 , *Eisenbarth SC, Williams A, Colegio OR, Meng H, Strowig T, Rongvaux A, Henao-Mejia J, Thaiss CA, Joly S, Gonzalez DG, Xu L, Zenewicz LA, Haberman AM, Elinav E, Kleinstein SH, Sutterwala FS, Flavell RA: NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Nature. 2012 Apr 25;484 (7395) :510-3, URL: https://pubmed.ncbi.nlm.nih.gov/22538615/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Isotype:

  Rat IgG2a kappa