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Anti-CLIC5 PE

CAT: 0270-1P-701-C100Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0270-1P-701-C100Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids) . These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Certification
RUO
Reactivity
Human
Immunogen
KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH) .
Target Antigen
CLIC5
Clone
CLIC5-02
Applications
FC-IC (QC tested)
Concentration
0.1 mg/mL
Format
PE
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References
*Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298 (6) :F1492-503., URL: http://www.ncbi.nlm.nih.gov/pubmed/20335315
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Isotype
Mouse IgG2b kappa

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