Anti-Drebrin PE

Anti-Drebrin PE

• Background: Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.
• Specifications: The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)
• Target Antigen: Drebrin
• Isotype: IgG2b
• Clone: DBN-N-03
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests. Intracellular staining.
• Validated Applications: FC
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug35(8):1111-3. Epub 2011 Apr 16., URL: https://pubmed.ncbi.nlm.nih.gov/21497902/,
• Other References: *Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1123(Pt 7):1160-70., URL: https://pubmed.ncbi.nlm.nih.gov/20215400/,*Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin-binding and actin-remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15253(2):673-80., URL: http://www.ncbi.nlm.nih.gov/pubmed/10585290, ,*Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 1516(22):7161-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/8929425, ,*Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar66(3):980-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/8769857, ,*Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25269(47):29928-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/7961990,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.