Anti-Hu Ig Kappa Light Chain FITC

• Description:

  Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa) . Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

• Certification:

  RUO

• Immunogen:

  Human IgG-kappa myeloma protein

• Target Antigen:

  Ig Kappa Light Chain

• Clone:

  TB28-2

• Applications:

  FC (QC tested)

• Format:

  FITC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15;138 (12) :4150-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/3495581, *Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992 Sep 15;89 (18) :8522-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1382292, *Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C; EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations. Haematologica. 2008 Apr;93 (4) :551-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18379010, *Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug;116 (2) :204-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/11488066, *Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10;114 (25) :5173-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/19846886

• Other References:

  *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa