Anti-Hu Ig Kappa Light Chain APC

Anti-Hu Ig Kappa Light Chain APC

• Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
• Specifications: The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human IgG-kappa myeloma protein
• Target Antigen: Ig Kappa Light Chain
• Isotype: Igg1 κ
• Clone: TB28-2
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests. Extracellular and intracellular staining.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15138(12):4150-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/3495581,*Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992 Sep 1589(18):8522-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1382292,*Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations. Haematologica. 2008 Apr93(4):551-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18379010,*Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug116(2):204-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/11488066,*Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10114(25):5173-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/19846886,
• Other References: *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.