Anti-Rt TCR alpha/beta FITC

Anti-Rt TCR alpha/beta FITC

• Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
• Specifications: The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Rat
• Immunogen: Rat T blasts and erythrocytes
• Target Antigen: TCR alpha/beta
• Isotype: Igg1 κ
• Clone: R73
• Applications: Flow cytometry: Recommended dilution: 1-3 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov26(11):2582-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8921942,*Kerstan A, Hünig T: Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells. J Immunol. 2004 Feb 1172(3):1341-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/14734708,*Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats. Am J Pathol. 2000 Jun156(6):2057-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/10854227,*Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191: J Immunol. 2004 Aug 15173(4):2849-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/15295004,*Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct72(4):1306-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/11603451,*Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr118(4):1405-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/18357346,*Hansson AS, Heinegård D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin-1). J Clin Invest. 1999 Sep104(5):589-98., URL: https://www.ncbi.nlm.nih.gov/pubmed/10487773,*Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7102(19):2426-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/11067799,*Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May16(5):655-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/15096488,*Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1169(1):73-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/2783336,*Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 1038(1):89-97., URL: https://pubmed.ncbi.nlm.nih.gov/19351909/,*Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Ther. 2000 Jul7(14):1238-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/10918493,*Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. J Endocrinol. 2007 Mar192(3):669-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/17332534,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.