Anti-Ms MHC Class II FITC

Anti-Ms MHC Class II FITC

• Background: MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
• Specifications: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse
• Immunogen: Activated C57BL/6 mouse spleen cells
• Target Antigen: MHC Class II
• Isotype: IgG2b
• Clone: M5/114
• Applications: Flow cytometry: Recommended dilution: 2-3 μg/mL.
• Validated Applications: FC
• Concentration: 0.5 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb8(2):143-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9491996?itool=,*Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec16(12):3661-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16221866?itool,*Kuwano Y, Pražma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug19(8):977-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/17804692?itool,*Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1174(3):1205-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/15661874?itool,*Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1155(1):53-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/11581285?itool,*De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct108 ( Pt 10):3219-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/7593283?itool=,*Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 2672(4):635-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/7679955?itool=,*Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec127(6):2488-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6170707?itool=,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.