Anti-Hu CD203c FITC

Anti-Hu CD203c FITC

• Background: CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
• Specifications: The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: HEK-293 cells transfected with human CD203c
• Target Antigen: CD203c
• Isotype: IgG1
• Clone: NP4D6
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests. Extracellular and intracellular staining.
• Validated Applications: FC
• Format: FITC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol. 2001 Dec126(4):335-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/11815741?itool,*Heneberg P, Riegerová K, Kučera P: Pimecrolimus is a potent inhibitor of allergic reactions to hymenopteran venom extracts and birch pollen allergen in vitro. PLoS One. 2015 Nov 1210(11):e0142953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=heneberg+p+riegerov%C3%A1+k+ku%C4%8Dera+p ,*Blatt K, Herrmann H, Mirkina I, Hadzijusufovic E, Peter B, Strommer S, Hoermann G, Mayerhofer M, Hoetzenecker K, Klepetko W, Ghanim V, Marth K, Füreder T, Wacheck V, Valenta R, Valent P: The PI3-kinase/mTOR-targeting drug NVP-BEZ235 inhibits growth and IgE-dependent activation of human mast cells and basophils. PLoS One. 20127(1):e29925. doi: 10.1371/journal.pone.0029925. Epub 2012 Jan 27., URL: https://www.ncbi.nlm.nih.gov/pubmed/22299028 ,
• Other References: *Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen-induced expression of CD203c on basophils predicts IgE-mediated wheat allergy. Allergol Int. 2009 Jun58(2):193-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/19240377?itool,*Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy. J Allergy Clin Immunol. 2009 Apr123(4):789-94.e20., URL: https://www.ncbi.nlm.nih.gov/pubmed/19348919?itool,*Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. Inflamm Res. 2009 Nov58(11):755-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/19418203?itool,*Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3. 1. Blood. 2001 May 1597(10):3303-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/11342463?itool,*Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999 Oct 194(7):2343-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/10498606?itool,*Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross-linking. Int J Immunopathol Pharmacol. 2008 Oct-Dec21(4):797-806., URL: https://www.ncbi.nlm.nih.gov/pubmed/19144265?itool,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.