Anti-GAPDHS FITC

CAT: 0270-1F-301-T050Size: 50 TestsDry Ice: NoHazardous: No
CAT#:0270-1F-301-T050Size:50 Tests
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Description
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Certification
RUO
Reactivity
Pig, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Target Antigen
GAPDHS
Clone
Hs-8
Applications
FC (QC tested)
Format
FITC
Buffer
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
References & Citations
*Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J: Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm. Reprod Biol Endocrinol. 2015 Mar 8;13:15. doi: 10.1186/s12958-015-0008-1., URL: https://www.ncbi.nlm.nih.gov/pubmed/25888749
Other References
*Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47 (5) :311-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12148547?dopt=, *Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65 (1) :28-32. , URL: https://www.ncbi.nlm.nih.gov/pubmed/10750294?dopt=, *Teplá O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999) ., URL:
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Applications Notes
6
Isotype
Mouse IgM

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