Rat IgG2b Isotype Control FITC

• Certification:

  RUO

• Clone:

  RTG2B1-2

• Applications:

  FC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  FITC

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Chen L, Arora M, Yarlagadda M, Oriss TB, Krishnamoorthy N, Ray A, Ray P: Distinct responses of lung and spleen dendritic cells to the TLR9 agonist CpG oligodeoxynucleotide. J Immunol. 2006 Aug 15;177 (4) :2373-83., URL: http://www.ncbi.nlm.nih.gov/pubmed/16887999 , *Mor A, Planer D, Luboshits G, Afek A, Metzger S, Chajek-Shaul T, Keren G, George J: Role of naturally occurring CD4+ CD25+ regulatory T cells in experimental atherosclerosis. Arterioscler Thromb Vasc Biol. 2007 Apr;27 (4) :893-900., URL: http://www.ncbi.nlm.nih.gov/pubmed/17272749 , *Koduru S, Wong E, Strowig T, Sundaram R, Zhang L, Strout MP, Flavell RA, Schatz DG, Dhodapkar KM, Dhodapkar MV: Dendritic cell-mediated activation-induced cytidine deaminase (AID) -dependent induction of genomic instability in human myeloma. Blood. 2012 Mar 8;119 (10) :2302-9., URL: https://pubmed.ncbi.nlm.nih.gov/22234692/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.

• Applications Notes:

  Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

• Isotype:

  Rat IgG2b