Mouse IgG2b Isotype Control Purified

Mouse IgG2b Isotype Control Purified

• Specifications: This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.
• Certification: RUO
• Host: Mouse
• Negative Species: Mouse, Rat, Pig, Human
• Isotype: IgG2b
• Clone: PLRV219
• Applications: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
• Validated Applications: FC, IP, IHC-P, IHC-F, ICC, WB, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Harald Schwarz, Jan Gornicec, Theresa Neuper, Maria Alejandra Parigiani, Michael Wallner, Albert Duschl, and Jutta Horejs-Hoeck: Biological Activity of Masked Endotoxin. Sci Rep. 2017 7: 44750. , URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC53577,
• Storage Conditions: Store at 2-8°C. Do not freeze.