Anti-Hu beta2-Microglobulin Biotin

• Description:

  Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

• Certification:

  RUO

• Immunogen:

  Purified isolated human beta2-microglobulin.

• Target Antigen:

  Beta2-Microglobulin

• Clone:

  B2M-02

• Applications:

  WB, IHC-P, ELISA, FC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98 (5) :1561-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11520808?dopt=, *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) . 1984;30 (6) :369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.

• Species:

  Pig, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1