Anti-Hu beta2-Microglobulin PE

Anti-Hu beta2-Microglobulin PE

• Background: Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Specifications: The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Negative Species: Rabbit, Dog, Mouse, Cow, Chicken
• Immunogen: Purified human beta2-microglobulin
• Target Antigen: beta2-Microglobulin
• Isotype: IgG2a
• Clone: B2M-01
• Applications: Flow cytometry: Recommended dilution: 1-2 μg/mL positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
• Validated Applications: FC
• Concentration: 0.1 mg/mL
• Format: PE
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb37(2):139-45., URL: https://pubmed.ncbi.nlm.nih.gov/23146557/,*Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec 79(24): 15226-15237., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13160,*Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep21(9):1257-65. Epub 2006 Jun 30., URL: https://www.ncbi.nlm.nih.gov/pubmed/16810512?dopt=,*Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 198430(6):369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A,*Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct30(14):1223-30., URL: https://pubmed.ncbi.nlm.nih.gov/7692238/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.