Anti-Hu CD54 Biotin

Anti-Hu CD54 Biotin

• Background: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Specifications: The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells the expression of CD54 is upregulated by activation.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Target Antigen: CD54
• Isotype: IgG2b
• Clone: 1H4
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC, WB, IHC-P, IHC-F, ICC
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar85(Pt 3):731-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/14993659?dopt=,
• Other References: *Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May85(9):3095-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3362863?dopt=A,*Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 1573(7):1896-903., URL: https://www.ncbi.nlm.nih.gov/pubmed/2469503?dopt=A,*Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2346(6283):425-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/1974032?dopt=A,*Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 1068(1):63-9. Erratum in: Cell 1992 Mar 668(5):following 994., URL: https://www.ncbi.nlm.nih.gov/pubmed/1346257?dopt=A,
• Storage Conditions: Store at 2-8°C. Do not freeze.