Anti-Hu CD54 Purified

Anti-Hu CD54 Purified

• Background: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Specifications: The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Cow, Rat
• Immunogen: Raji human Burkitt's lymphoma cell line
• Target Antigen: CD54
• Isotype: IgG2a
• Clone: MEM-111
• Applications: Flow cytometry: Recommended dilution: 0.5-4 μg/mL. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL positive tissue: thymus, RE cells. Western blotting: Recommended dilution: 1-2 μg/mL non-reducing conditions.
• Validated Applications: WB, IHC-P, ELISA, FC, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998)., URL: https://www.ncbi.nlm.nih.gov/pubmed/9737670?dopt=A,*Tachimoto H. et al., Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases. Am J Respir Cell Mol Biol 26, 645 (2002)., URL: https://pubmed.ncbi.nlm.nih.gov/12034562/,*Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003)., URL: https://www.physiology.org/doi/full/10.1152/ajpcel,*Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003)., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC16478,*Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun21(11):1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10817270?ordin,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15179(12):8128-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056355,*Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun21(11):1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10602070?ordin,*Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992)., URL: https://www.ncbi.nlm.nih.gov/pubmed/1283598?dopt=A,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15179(12):8128-36., URL: https://pubmed.ncbi.nlm.nih.gov/18056355/,
• Other References: *Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May85(9):3095-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3362863?dopt=A,*Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 1573(7):1896-903., URL: https://www.ncbi.nlm.nih.gov/pubmed/2469503?dopt=A,*Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2346(6283):425-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/1974032?dopt=A,*Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 1068(1):63-9. Erratum in: Cell 1992 Mar 668(5):following 994., URL: https://www.ncbi.nlm.nih.gov/pubmed/1346257?dopt=A,
• Storage Conditions: Store at 2-8°C. Do not freeze.