Anti-Hu CD79a APC

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta) . They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR) . CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Non-human primates, Human

Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a

CD79a

HM47

FC-IC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128 (2) :306-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=bhargava+p+ross+js+azumi+n, *Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147 (11) :2474-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/1747162?ordinal, *Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95 (8) :2725-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/10753858 , *Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42 (4) :437-45., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=fernandez+nj+west+kh , *Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126 (7) :862-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/12123231 , *Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1 (1) :65-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hassan+a+perry+a+ning+y , *Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78 (24) :13891-900., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=netto+g+garcia+jv+cravens+pd

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a) - and Igbeta (CD79b) -mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177 (11) :7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina, *Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172 (4) :2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina, *Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194 (11) :1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

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