Anti-Hu CD79a PE

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta) . They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR) . CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Human

IgM complex isolated from Daudi cells

CD79a

ZL7.4

FC (QC tested)

PE

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Stopforth RJ, Oldham RJ, Tutt AL, Duriez P, Chan HTC, Binkowski BF, Zimprich C, Li D, Hargreaves PG, Cong M, Reddy V, Leandro MJ, Cambridge G, Lux A, Nimmerjahn F, Cragg MS: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB. J Immunol. 2018 Mar 1;200 (5) :1937-1950., URL: https://pubmed.ncbi.nlm.nih.gov/29351998/, *Bell PB, Rooney N, Bosanquet AG: CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase. Cytometry. 1999 Jun 15;38 (3) :102-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/10397328, *Luger D, Yang YA, Raviv A, Weinberg D, Banerjee S, Lee MJ, Trepel J, Yang L, Wakefield LM: Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects. PLoS One. 2013 Oct 16;8 (10) :e76115., URL: https://www.ncbi.nlm.nih.gov/pubmed/24146823, *Cragg MS, Chan HT, Fox MD, Tutt A, Smith A, Oscier DG, Hamblin TJ, Glennie MJ: The alternative transcript of CD79b is overexpressed in B-CLL and inhibits signaling for apoptosis. Blood. 2002 Nov 1;100 (9) :3068-76., URL: https://pubmed.ncbi.nlm.nih.gov/12384401/

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a) - and Igbeta (CD79b) -mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177 (11) :7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina, *Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174 (3) :1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina, *Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173 (2) :1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina, *Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172 (4) :2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina, *Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194 (11) :1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody ZL7.4 interacts with extracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

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