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Anti-Hu CD95 APC

CAT: 0270-1A-362-T100Size: 100 TestsDry Ice: NoHazardous: No
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CAT#:0270-1A-362-T100Size:100 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Certification
RUO
Reactivity
Human
Immunogen
HUT-78 human T cell lymphoma cell line
Target Antigen
CD95
Clone
LT95
Applications
FC (QC tested)
Format
APC
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005 Jun 17;280 (24) :22856-67. Epub 2005 Mar 8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15757891?dopt=
Other References
*Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314 (3) :554-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/18078929?ordin, *Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106 (6) :2033-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/15941911?ordin, *Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170 (12) :6209-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/12794152?ordin, *Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17 (6) :1675-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/9501089?ordina
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Applications Notes
6
Isotype
Mouse IgG1

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