Anti-Hu CD122 APC

• Description:

  CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex.

• Certification:

  RUO

• Immunogen:

  TL-Mor cell line

• Target Antigen:

  CD122

• Clone:

  TU27

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Elkins J, Sheridan J, Amaravadi L, Riester K, Selmaj K, Bielekova B, Parr E, Giovannoni G: CD56 (bright) natural killer cells and response to daclizumab HYP in relapsing-remitting MS. Neurol Neuroimmunol Neuroinflamm. 2015 Jan 22;2 (2) :e65., URL: http://www.ncbi.nlm.nih.gov/pubmed/25635261 , *Gallart T, de la Fuente MA, Barceló JJ, Alberola-Ila J, Lozano F: Desialylation of T lymphocytes overcomes the monocyte dependency of pokeweed mitogen-induced T-cell activation. Immunology 1997 Jan;90 (1) :57-65., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=desialylation+of+t+lymphocytes+overcomes+the+monocyte+dependency+of+pokeweed , *Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A: Tissue-Specific Education of Decidual NK Cells. J Immunol. 2015 Oct 1;195 (7) :3026-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/26320253 , *Ozawa A, Tada H, Tamai R, Uehara A, Watanabe K, Yamaguchi T, Shimauchi H, Takada H, Sugawara S: Expression of IL-2 receptor beta and gamma chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2. J Leukoc Biol. 2003 Sep;74 (3) :352-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/12949238 , *Sahraoui Y, Perraki M, Theodoropoulou M, Allouche M, Tsapis A, Ammar A, Clemenceau C, Bokogiorgou S, Yacci T, Katrinakis G, Jasmin C, Georgoulias V: Autocrine IL-2-dependent growth of a newly established CD3+, CD16-, CD56+, CD57+, J (H) -, TCRbeta-, TCRgamma- leukemia cell line (NOI-90) . Leukemia. 1997 Feb;11 (2) :245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/9009088 , *Huss DJ, Mehta DS, Sharma A, You X, Riester KA, Sheridan JP, Amaravadi LS, Elkins JS, Fontenot JD: In vivo maintenance of human regulatory T cells during CD25 blockade. J Immunol. 2015 Jan 1;194 (1) :84-92., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=huss+dj+mehta+ds+sharma+a

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa