Anti-Hu CD11b Purified Low Endotoxin

Anti-Hu CD11b Purified Low Endotoxin

• Background: CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
• Specifications: The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: Rheumatoid synovial cells and human monocytes.
• Target Antigen: CD11b
• Isotype: IgG1
• Clone: ICRF44
• Applications: Functional application: Blocking of granulocyte activation. Flow cytometry: Recommended dilution: 1-12 µg/mL
• Validated Applications: FC, IP, IHC-F, ICC, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Low Endotoxin
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor 946 Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15197(6):2229-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/27534550 ,*Hua J, Sakamoto K, Nagaoka I: Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils. J Leukoc Biol. 2002 Apr71(4):632-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/11927650,*Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol. 2001 Dec13(12):1571-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/11717198,*Masten BJ, Olson GK, Tarleton CA, Rund C, Schuyler M, Mehran R, Archibeque T, Lipscomb MF: Characterization of myeloid and plasmacytoid dendritic cells in human lung. J Immunol. 2006 Dec 1177(11):7784-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/17114449,*Tieu BC, Lee C, Sun H, Lejeune W, Recinos A 3rd, Ju X, Spratt H, Guo DC, Milewicz D, Tilton RG, Brasier AR: An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice. J Clin Invest. 2009 Dec119(12):3637-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/19920349,*Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 1597(10):2932-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/11342414,
• Other References: *Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 195(3):911-20., URL: https://pubmed.ncbi.nlm.nih.gov/10648403/,*Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15119(3-4):287-98., URL: https://pubmed.ncbi.nlm.nih.gov/17628696/,*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 200743(8):597-606., URL: https://pubmed.ncbi.nlm.nih.gov/17895634/,
• Storage Conditions: Store at 2-8°C. Do not freeze.