Anti-Hu CD11b Purified Azide Free

Anti-Hu CD11b Purified Azide Free

• Background: CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
• Specifications: The antibody MEM-170 recognizes an extracellular epitope of CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human granulocytes
• Target Antigen: CD11b
• Isotype: IgG1
• Clone: MEM-170
• Applications: Functional application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen. Flow cytometry: Recommended dilution: 1 μg/mL.
• Validated Applications: FC, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Azide Free
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor 946 Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15197(6):2229-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/27534550,
• Other References: *Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 195(3):911-20., URL: https://pubmed.ncbi.nlm.nih.gov/10648403/,*Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15119(3-4):287-98., URL: https://pubmed.ncbi.nlm.nih.gov/17628696/,*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 200743(8):597-606., URL: https://pubmed.ncbi.nlm.nih.gov/17895634/,
• Storage Conditions: Store at 2-8°C. Do not freeze.