Anti-DDIT4L Purified

• Description:

  DDIT4L (DNA-damage-inducible transcript 4-like), also known as REDD2 (regulated in development and DNA damage response 2) or RTP801L is a stress-inducted protein, which was shown to mediate monocyte cell death through a reduction in thioredoxin-1 expression, and is highly expressed in atherosclerotic lesions. Stimulation of DDIT4L expression in macrophages increases oxidized LDL-induced macrophage death.

• Certification:

  RUO

• Immunogen:

  N-terminal recombinant fragment of human DDIT4L (amino acids 2-98)

• Target Antigen:

  DDIT4L

• Clone:

  DDIT-03

• Applications:

  WB, FC-IC

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Other References:

  *Cuaz-Pérolin C, Furman C, Larigauderie G, Legedz L, Lasselin C, Copin C, Jaye M, Searfoss G, Yu KT, Duverger N, Nè gre-Salvayre A, Fruchart JC, Rouis M.REDD2 gene is upregulated by modified LDL or hypoxia and mediates human macrophage cell death. Arterioscler Thromb Vasc Biol. 2004 Oct;24 (10) :1830-5., URL: https://pubmed.ncbi.nlm.nih.gov/15308555/, *Corradetti MN, Inoki K, Guan KL: The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem. 2005 Mar 18;280 (11) :9769-72. , URL: http://www.ncbi.nlm.nih.gov/pubmed/15632201, , *Imen JS, Billiet L, Cuaz-Pérolin C, Michaud N, Rouis M: The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression. Free Radic Biol Med. 2009 May 15;46 (10) :1404-10, URL: https://pubmed.ncbi.nlm.nih.gov/19268525/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody DDIT-03 recognizes DDIT4L / REDD2 protein, which belongs to intracellular stress-induced proteins involved in mediation of cell death.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1