Anti-NCK1 Purified

Anti-NCK1 Purified

• Background: NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.
• Specifications: The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Mouse, Human
• Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST
• Target Antigen: NCK1
• Isotype: IgG1
• Clone: EM-06
• Applications: Western blotting: Recommended dilution: 2 μg/mL positive control: Jurkat cell lysate. Immunocytochemistry: Recommended dilution: 2 μg/mL positive control: human NCK1-transfected COS-7 cells.
• Validated Applications: WB, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Borroto A, Arellano I, Dopfer EP, Prouza M, Suchánek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1190(3):1103-12., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=borroto+a+arellano+i+prouza+m,
• Storage Conditions: Store at 2-8°C. Do not freeze.