Anti-Ms CD80 Purified

Anti-Ms CD80 Purified

• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Specifications: The Armenian hamster monoclonal antibody 16-10A1 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Armenian Hamster
• Species Reactivity: Mouse, Dog
• Immunogen: CD80-transfected CHO cell line
• Target Antigen: CD80
• Isotype: Igg2
• Clone: 16-10A1
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IP, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Horspool JH, Perrin PJ, Woodcock JB, Cox JH, King CL, June CH, Harlan DM, St Louis DC, Lee KP: Nucleic acid vaccine-induced immune responses require CD28 costimulation and are regulated by CTLA4. J Immunol. 1998 Mar 15160(6):2706-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/9510170?itool=,*Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L: CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis. J Immunol. 2002 Sep 15169(6):3180-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/12218136?itool,*Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, Holdsworth SR: Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis. J Am Soc Nephrol. 2005 Jul16(7):2012-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/15944341?itool,*Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P: Induction of B7-1 in podocytes is associated with nephrotic syndrome. J Clin Invest. 2004 May113(10):1390-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/15146236?itool,*Paust S, Lu L, McCarty N, Cantor H: Engagement of B7 on effector T cells by regulatory T cells prevents autoimmune disease. Proc Natl Acad Sci U S A. 2004 Jul 13101(28):10398-403. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15235129?itool,*Quah B, Ni K, O'Neill HC: In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity. Int Immunol. 2004 Apr16(4):567-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/15039387?itool,*Mukherjee S, Ahmed A, Malu S, Nandi D: Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2. J Leukoc Biol. 2006 Jul80(1):66-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16624934?itool,*Gueirard P, Ave P, Balazuc AM, Thiberge S, Huerre M, Milon G, Guiso N: Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun. 2003 Jul71(7):4137-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/12819105?itool,
• Storage Conditions: Store at 2-8°C. Do not freeze.