Anti-Ms CD86 Purified

Anti-Ms CD86 Purified

• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Specifications: The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse
• Immunogen: LPS-activated CBA/Cs mouse splenic B cells
• Target Antigen: CD86
• Isotype: IgG2a κ
• Clone: GL-1
• Applications: Flow cytometry: Recommended dilution: 2 μg/mL positive control: murine splenocytes.
• Validated Applications: FC, IP, IHC-F, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005 Feb54(2):434-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/15677501?itool,*Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS One. 2009 Aug 124(8):e6600., URL: https://www.ncbi.nlm.nih.gov/pubmed/19672303?itool,*Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures. Blood. 2000 Nov 196(9):3029-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/11049981?itool,*Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am J Respir Crit Care Med. 2008 Feb 1177(3):301-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17989345?itool,*Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells. J Am Soc Nephrol. 2008 Mar19(3):515-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/18184859?itool,*Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science. 1993 Nov 5262(5135):905-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7694361?itool=,*Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells. J Exp Med. 1999 Sep 20190(6):749-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/10499913?itool,*Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J Immunol. 2008 Dec 1181(11):7863-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/19017976?itool,*Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness. J Immunol. 2003 Aug 15171(4):1758-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902475?itool,
• Storage Conditions: Store at 2-8°C. Do not freeze.