Anti-AGR3 Purified

Anti-AGR3 Purified

• Background: AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
• Specifications: The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa) a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Purified human AGR3 protein
• Target Antigen: AGR3
• Isotype: IgG1
• Clone: AGR3.1
• Applications: Immunohistochemistry (paraffin sections): Recommended dilution: 1 μg/mL positive tissue: breast cancer. Immunohistochemistry (frozen sections): Recommended dilution: 1 μg/mL positive tissue: breast cancer. Western blotting: Recommended dilution: 1 μg/mL positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line. Immunocytochemistry: Recommended dilution: 1 μg/mL positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
• Validated Applications: WB, IHC-P, IHC-F, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Obacz J, Brychtová V, Podhorec J, Fabian P, Dobeš P, Vojtěšek B, Hrstka R: Anterior gradient protein 3 is associated with less aggressive tumors and better outcome of breast cancer patients. Onco Targets Ther. 2015 Jun 248:1523-32., URL: https://pubmed.ncbi.nlm.nih.gov/26170690/,
• Storage Conditions: Store at 2-8°C. Do not freeze.