LC3 A/B rabbit pAb

LC3 A/B rabbit pAb

• Description: Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. similarity: Belongs to the MAP1 LC3 family. subcellular location: LC3-II binds to the autophagic membranes. subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. tissue specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
• UniProt: Q9H492/Q9GZQ8
• Cellular Locus: Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor. Endomembrane system ; Lipid-anchor. Cytoplasm, cytoskeleton. LC3-II binds to the autophagic membranes..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from human LC3 A/B
• Clonality: Polyclonal
• Validated Applications: WB, ELISA, IHC
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Molecular Weight: 14,16kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.