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CD66e Mouse Monoclonal Antibody

CAT: 0013-GTR18155179-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18155179-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD66e Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody .Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
CEA; Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e
UniProt
P06731
Reactivity
Human
Target
CEACAM5
Clonality
Monoclonal
Clone
BED52
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
The antibody was purified by affinity chromatography.
Dilution
IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG1 kappa
Entrez
1048

UniProtKB · P06731

Cell adhesion molecule CEACAM5

CEAM5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P06731
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CEACAM5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
H9KVA7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCell adhesion

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

Immunoglobulin domainRepeatSignal

Disease

Oncogene

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