MMP8 (Cleaved-Leu101) rabbit pAb

• Background:

  Catalytic activity:Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I., cofactor:Binds 2 zinc ions per subunit., cofactor:Binds 3 calcium ions per subunit., domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme., enzyme regulation:Cannot be activated without removal of the activation peptide., function:Can degrade fibrillar type I, II, and III collagens., similarity:Belongs to the peptidase M10A family., similarity:Contains 4 hemopexin-like domains., subcellular location:Stored in intracellular granules., tissue specificity:Neutrophils.

• Description:

  Catalytic activity: Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3. 4. 24. 7, this enzyme cleaves type III collagen more slowly than type I. Cofactor: Binds 2 zinc ions per subunit. Cofactor: Binds 3 calcium ions per subunit. Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. enzyme regulation: Cannot be activated without removal of the activation peptide. function: Can degrade fibrillar type I, II, and III collagens. similarity: Belongs to the peptidase M10A family. similarity: Contains 4 hemopexin-like domains. subcellular location: Stored in intracellular granules. tissue specificity: Neutrophils.

• Synonyms:

  Neutrophil collagenase (EC 3.4.24.34;Matrix metalloproteinase-8;MMP-8;PMNL collagenase;PMNL-CL)

• Gene ID:

  4317

• UniProt:

  P22894

• Cellular Locus:

  Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Stored in intracellular granules.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Synthesized peptide derived from human MMP8 (Cleaved-Leu101)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, IHC

• Validated Applications:

  WB, IHC

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-2000; IHC-p 1:50-300

• Molecular Weight:

  40 53kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  40 - 53 kD

• Subcellular Location:

  Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

• Other Product Names:

  Neutrophil collagenase (EC 3.4.24.34; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL)

• Gene ID (Human):

  4317

• SwissProt (Human):

  P22894