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APOE Antibody (Biotin)

CAT: 0013-GTR18144342Size: 0.05 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144342Size:0.05 mg
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Description
APOE Antibody (Biotin)
Product Name Alternative
AD2, LPG, LDLCQ5Apolipoprotein EApo-E
UniProt
P02649
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography and then biotinylated. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA
Target
APOE
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Metabolism Research
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
ApoE3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Sandwich:To detect hApoE3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human ApoE3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Western Blot:To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing
Tested Applications
ELISA, WB
NCBI Accession Number
P02649
Host or Source
Rabbit

UniProtKB · P02649

Apolipoprotein E

APOE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
B2RC15, C0JYY5, Q9P2S4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Alzheimer diseaseAmyloidosisDisease variantHyperlipidemiaNeurodegeneration

Biological process

Cholesterol metabolismHost-virus interactionLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

PTM

GlycationGlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Ligand

Lipid-binding

Domain

RepeatSignal

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