Anti-Sphingosine Kinase type 1 Antibody

Anti-Sphingosine Kinase type 1 Antibody

• Description: Rabbit Polyclonal Antibody specific to Sphingosine Kinase Type 1
• Product Name Alternative: SK 1, SPK 1, EC 2.7.1.91, Acetyltransferase SPHK1, EC 2.3.1.-
• CAS Number: 9007-83-4
• Gene Name: SPHK1
• NCBI Gene ID: SPHK1
• UniProt: Q9NYA1
• Accession Number: NP_001136073
• Cellular Locus: Cytoplasm, Nucleus, Cell membrane, Endosome membrane, Membrane, clathrin-coated pit, Cell junction, synapse
• Host: Rabbit
• Reactivity: Human
• Immunogen: Synthetic peptide conjugated to KLH.
• Target Antigen: Sphingosine kinase 1
• Target: Sphingosine Kinase type 1
• Clonality: Polyclonal
• Type: Antibody
• Applications: IHC, IHC-P
• Field of Research: Enzymes
• Purification Method: Purified by peptide immuno-affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:20577214, PubMed:23602659, PubMed:29662056, PubMed:24929359, PubMed:11923095). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed:23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:28049734, PubMed:24929359). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698). {PubMed:11923095, PubMed:16118219, PubMed:20577214, PubMed:23602659, PubMed:23935096, PubMed:24929359, PubMed:25417698, PubMed:28049734, PubMed:29662056}.; Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. {UniProtKB:Q8CI15}.
• Additionnal Information:
  
  Immunohistochemistry: Use at 10ug/ml.
• Storage Conditions: This antibody is stable at 4°C (short- term) and -70°C (long-term).
• Specificity: This antibody recognizes an epitope within the N-terminal domain of human SPHK1.
• Formulation: PBS containing 0.1% sodium azide
• Target Background: Sphingosine kinase (SPHK) phosphorylates sphingosine to form a bioactive lipid, sphingosine 1-phosphate (S1P). S1P is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival while extracellularly it is a ligand for endothelial differentiation gene 1 (EDG1). Expression of SPHK1 and subsequent production of S1P promotes the G1-S transition, protects cells from apoptosis, stimulates migration and angiogenesis, and induces tumor formation in mice.