CASP3 antibody

• Background:

  Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.

• Synonyms:

  Caspase-3 (CASP-3) |Apopain|Cysteine protease CPP32 (CPP-32) |Protein Yama|SREBP cleavage activity 1 (SCA-1) |Caspase-3 subunit p17|Caspase-3 subunit p12|CASP3|CPP32

• UniProt:

  P42574

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Caspase 3, apoptosis-related cysteine peptidase

• Target:

  CASP3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, IF

• Field of Research:

  Cancer, Metabolism, Neuroscience

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  32-35 kDa, 17 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, IF

• Immunogen Sequence:

  29-175aa