RELA antibody

• Background:

  Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel, p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

• Synonyms:

  Transcription factor p65|Nuclear factor NF-kappa-B p65 subunit|Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|RELA|NFKB3

• Gene ID:

  5970

• UniProt:

  Q04206

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  V-rel reticuloendotheliosis viral oncogene homolog A (avian)

• Target:

  RELA

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IHC, IF, WB

• Field of Research:

  Metabolism, Immunology, Cancer, Stem Cells, Cell Division and Proliferation

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  65 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IHC, IF, WB

• Immunogen Sequence:

  1-220aa