UPF3A antibody

• Background:

  Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.

• Synonyms:

  Regulator of nonsense transcripts 3A|Nonsense mRNA reducing factor 3A|Up-frameshift suppressor 3 homolog A (hUpf3) |UPF3A|RENT3A|UPF3

• Gene ID:

  65110

• UniProt:

  Q9H1J1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  UPF3 regulator of nonsense transcripts homolog A (yeast)

• Target:

  UPF3A

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:5000; IHC:1:20-1:200; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  51 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC