UIMC1 antibody

• Background:

  Ubiquitin-binding protein (PubMed:24627472) . Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.36) . Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) . The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

• Synonyms:

  BRCA1-A complex subunit RAP80|Receptor-associated protein 80|Retinoid X receptor-interacting protein 110|Ubiquitin interaction motif-containing protein 1|UIMC1|RAP80|RXRIP110

• Gene ID:

  51720

• UniProt:

  Q96RL1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Ubiquitin interaction motif containing 1

• Target:

  UIMC1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IF, IHC,IF

• Field of Research:

  Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:1000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  97 kDa, 80 kDa, 71-75 kDa, 61 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IF, IHC, IF