UIMC1 antibody

• Background :

  Ubiquitin-binding protein (PubMed:24627472) . Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.36) . Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) . The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

• Synonyms :

  BRCA1-A complex subunit RAP80|Receptor-associated protein 80|Retinoid X receptor-interacting protein 110|Ubiquitin interaction motif-containing protein 1|UIMC1|RAP80|RXRIP110

• Gene ID :

  51720

• UniProt :

  Q96RL1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Ubiquitin interaction motif containing 1

• Target :

  UIMC1

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IF, IHC,IF

• Field of Research :

  Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:200-1:1000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  97 kDa, 80 kDa, 71-75 kDa, 61 kDa

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IF, IHC, IF