TP53 antibody

• Background:

  Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492) .

• Synonyms:

  Cellular tumor antigen p53|Antigen NY-CO-13|Phosphoprotein p53|Tumor suppressor p53|TP53|P53

• Gene ID:

  94241

• UniProt:

  P04637

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Tumor protein p53

• Target:

  TP53

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IHC, IP, WB, IF

• Field of Research:

  Cancer

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  53 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IHC, IP, WB, IF