RUNX1 antibody

RUNX1 antibody

• Background: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells(Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells(PubMed:17377532).
• Synonyms: Runt-related transcription factor 1|Acute myeloid leukemia 1 protein|Core-binding factor subunit alpha-2 (CBF-alpha-2)|Oncogene AML-1|Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B, PEBP2-alpha B)|SL3-3 enhancer factor 1 alpha B subunit|SL3/AKV core-binding factor alpha B subunit|RUNX1|AML1|CBFA2
• CAS Number: 9007-83-4
• Gene ID: 861
• UniProt: Q01196
• Host: Rabbit
• Reactivity: Human, Mouse
• Immunogen: runt-related transcription factor 1
• Target: RUNX1
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA, WB, IF
• Field of Research: Stem Cells, cancer, Neuroscience, Developmental biology
• Purification: Immunogen affinity purified
• Dilution: WB: 1:500-1:2000; IF: 1:20-1:200
• Purity: ≥95% as determined by SDS-PAGE
• Form: Liquid
• Molecular Weight: 48-55 kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)