RICTOR antibody

• Background:

  Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

• Synonyms:

  Rapamycin-insensitive companion of mTOR|AVO3 homolog (hAVO3) |RICTOR|KIAA1999

• Gene ID:

  253260

• UniProt:

  Q6R327

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Rapamycin-insensitive companion of mTOR

• Target:

  RICTOR

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Developmental biology

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:1000-1:5000; IHC: 1:50-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  192 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC

• Immunogen Sequence:

  1-60AA