PNPT1 antibody

RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

Polyribonucleotide nucleotidyltransferase 1, mitochondrial|3'-5' RNA exonuclease OLD35|PNPase old-35|Polynucleotide phosphorylase 1 (PNPase 1) |Polynucleotide phosphorylase-like protein|PNPT1|PNPASE

87178

Q8TCS8

Human, Mouse, Rat

Polyribonucleotide nucleotidyltransferase 1

PNPT1

ELISA, WB, IHC, IP

Epigenetics, Metabolism

Immunogen affinity purified

WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

≥95% as determined by SDS-PAGE

Liquid

90 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)