NGEF antibody

• Background :

  Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension (By similarity) .

• Synonyms :

  Ephexin-1|Eph-interacting exchange protein|Neuronal guanine nucleotide exchange factor|NGEF

• Gene ID :

  25791

• UniProt :

  Q8N5V2

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Neuronal guanine nucleotide exchange factor

• Target :

  NGEF

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IP, IHC

• Field of Research :

  Developmental biology

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:50-1:500

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  75 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IP, IHC