NEK2 antibody

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434) . NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143) . Involved in the regulation of centrosome disjunction (PubMed:26220856) . Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

Serine/threonine-protein kinase Nek2|HSPK 21|Never in mitosis A-related kinase 2 (NimA-related protein kinase 2) |NimA-like protein kinase 1|NEK2|NEK2A|NLK1

P51955

Human, Mouse, Rat

NIMA (never in mitosis gene a) -related kinase 2

NEK2

ELISA, WB, IHC

Metabolism

Immunogen affinity purified

WB: 1:500-1:2000; IHC: 1:20-1:200

≥95% as determined by SDS-PAGE

Liquid

48 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)