MAGEA3 antibody

• Background:

  Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

• Synonyms:

  Melanoma-associated antigen 3|Antigen MZ2-D|Cancer/testis antigen 1.3 (CT1.3) |MAGE-3 antigen|MAGEA3|MAGE3

• Gene ID:

  4102

• UniProt:

  P43357

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Melanoma antigen family A, 3

• Target:

  MAGEA3

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Cancer, Developmental biology

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:2000; IHC: 1:50-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  35-55 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  4E9

• Tested Applications:

  ELISA, WB, IHC