MAGEA3 antibody

• Background :

  Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

• Synonyms :

  Melanoma-associated antigen 3|Antigen MZ2-D|Cancer/testis antigen 1.3 (CT1.3) |MAGE-3 antigen|MAGEA3|MAGE3

• Gene ID :

  4102

• UniProt :

  P43357

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Melanoma antigen family A, 3

• Target :

  MAGEA3

• Clonality :

  Monoclonal

• Isotype :

  IgG2a

• Applications :

  ELISA, WB, IHC

• Field of Research :

  Cancer, Developmental biology

• Purification :

  Protein A+G purification

• Dilution :

  WB: 1:500-1:2000; IHC: 1:50-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  35-55 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID :

  4E9

• Tested Applications :

  ELISA, WB, IHC