ISCU antibody

• Background :

  Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020) . First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity) .

• Synonyms :

  Iron-sulfur cluster assembly enzyme ISCU|NifU-like N-terminal domain-containing protein|NifU-like protein|ISCU|NIFUN

• Gene ID :

  23479

• UniProt :

  Q9H1K1

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Iron-sulfur cluster scaffold homolog (E. coli)

• Target :

  ISCU

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IHC, IF, IP

• Field of Research :

  Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  14-15 kDa, 18-19 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IHC, IF, IP