GRK5 antibody

• Background:

  Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs) . Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) .

• Synonyms:

  G protein-coupled receptor kinase 5|G protein-coupled receptor kinase GRK5|GRK5|GPRK5

• Gene ID:

  2869

• UniProt:

  P34947

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  G protein-coupled receptor kinase 5

• Target:

  GRK5

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB

• Field of Research:

  Neuroscience, Cardiovascular, Metabolism, Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  68 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB