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EIF4A3 antibody

CAT: 0710-FNab02718Size: 100 µgDry Ice: NoHazardous: No
CAT#:0710-FNab02718Size:100 µg
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Background
ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD) . Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes) ; specifically inhibits formation of proapoptotic isoforms such as Bcl-X (S) ; the function is different from the established EJC assembly. Involved in craniofacial development.
Synonyms
Eukaryotic initiation factor 4A-III (eIF-4A-III, eIF4A-III) |ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD box protein 48|Eukaryotic initiation factor 4A-like NUK-34|Eukaryotic translation initiation factor 4A isoform 3|Nuclear matrix protein 265 (NMP 265, hNMP 265) |Eukaryotic initiation factor 4A-III, N-terminally processed|EIF4A3|DDX48|KIAA0111
Gene ID
9775
UniProt
P38919
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Eukaryotic translation initiation factor 4A, isoform 3
Target
EIF4A3
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IP
Field of Research
Neuroscience, Cancer, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:2000; IP: 1:200-1:2000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
47 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IP

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