E2F8 antibody

• Background :

  Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3) . Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.

• Synonyms :

  Transcription factor E2F8 (E2F-8) |E2F8

• Gene ID :

  79733

• UniProt :

  A0AVK6

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  E2F transcription factor 8

• Target :

  E2F8

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IP

• Field of Research :

  Signal Transduction, Metabolism, Developmental biology

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:2000; IP: 1:200-1:2000

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  82 kDa, 105 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IP