E2F1 antibody

• Background:

  Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812) .

• Synonyms:

  Transcription factor E2F1 (E2F-1) |PBR3|Retinoblastoma-associated protein 1 (RBAP-1) |Retinoblastoma-binding protein 3 (RBBP-3) |pRB-binding protein E2F-1|E2F1|RBBP3

• Gene ID:

  1869

• UniProt:

  Q01094

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E2F transcription factor 1

• Target:

  E2F1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IHC, WB

• Field of Research:

  Cell Division and Proliferation, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IHC: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  55 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IHC, WB