DTL antibody

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX (DTL) complex, also named CRL4 (CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21 (CIP1), FBXO18/FBH1 and KMT5A (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613) . CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480) . CDKN1A/p21 (CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548) . KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445) . Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX (DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX (DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613) .

Denticleless protein homolog|DDB1- and CUL4-associated factor 2|Lethal (2) denticleless protein homolog|Retinoic acid-regulated nuclear matrix-associated protein|DTL|CDT2|CDW1|DCAF2|L2DTL|RAMP

51514

Q9NZJ0

Human, Mouse, Rat

Denticleless homolog (Drosophila)

DTL

ELISA, WB, IP

Cell Division and Proliferation, Metabolism

Immunogen affinity purified

WB: 1:500-1:2000; IP: 1:200-1:2000

≥95% as determined by SDS-PAGE

Liquid

79 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)