CUL4A antibody

• Background:

  Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX (DET1-COP1) directs ubiquitination of JUN. DCX (DDB2) directs ubiquitination of XPC. DCX (DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX (DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX (DTL) directs autoubiquitination of DTL.

• Synonyms:

  Cullin-4A (CUL-4A) |CUL4A

• Gene ID:

  8451

• UniProt:

  Q13619

• Host:

  Mouse

• Reactivity:

  Human, Monkey, Mouse, Rat

• Immunogen:

  Cullin 4A

• Target:

  CUL4A

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB, IHC, IP, IF

• Field of Research:

  Metabolism

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  88 kDa, 77 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  5A7

• Tested Applications:

  ELISA, WB, IHC, IP, IF

• Immunogen Sequence:

  460-759aa