BUB3 antibody

• Background:

  Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

• Synonyms:

  Mitotic checkpoint protein BUB3|BUB3

• Gene ID:

  9184

• UniProt:

  O43684

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Budding uninhibited by benzimidazoles 3 homolog (yeast)

• Target:

  BUB3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IF

• Field of Research:

  Cell Division and Proliferation

• Purification:

  Immunogen affinity purified

• Dilution:

  IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IF